Cell segmentation is a major bottleneck in extracting quantitative single-cell information from microscopy data. The challenge is exasperated in the setting of microstructured environments. While deep learning approaches have proven useful for general cell segmentation tasks, previously available segmentation tools for the yeast-microstructure setting rely on traditional machine learning approaches. Here we present convolutional neural networks trained for multiclass segmenting of individual yeast cells and discerning these from cell-similar microstructures. An U-Net based semantic segmentation approach, as well as a direct instance segmentation approach with a Mask R-CNN are demonstrated. We give an overview of the datasets recorded for training, validating and testing the networks, as well as a typical use-case. We showcase the methods' contribution to segmenting yeast in microstructured environments with a typical systems or synthetic biology application. The models achieve robust segmentation results, outperforming the previous state-of-the-art in both accuracy and speed. The combination of fast and accurate segmentation is not only beneficial for a posteriori data processing, it also makes online monitoring of thousands of trapped cells or closed-loop optimal experimental design feasible from an image processing perspective. Code is and data samples are available at https://git.rwth-aachen.de/bcs/projects/tp/multiclass-yeast-seg.

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Journal Article

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Prangemeier T, Wildner C, Françani AO, Reich C, Koeppl H

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