Oil palm (Elaeis guineensis) trunk chips were processed by steam explosion under different steam conditions, followed by alkaline extraction and fermentation to produce efficient lignocellulosic ethanol as sustainable alternative energy resource. The optimum condition of steam explosion was attained at 210°C for 4 min (α-cellulose: 58.83% and lignin: 27.12%). Taguchi 3 factor design [(sodium hydroxide concentration (NaOH), temperature and time)] was performed to optimize alkaline extraction. The optimum condition at 15% NaOH, 90°C for 60 min gave highest percentage α-cellulose: 87.14% and lowest percentage of lignin: 6.13%. Simultaneous saccharification and fermentation (SSF) involved 10% dry weight pretreated fibers, Celluclast 1.5L (15 FPU /gram substrate), Novozyme 188 (15 IU/gram substrate) and Saccharomyces cerevisiae SC90. The highest ethanol concentration (CP) produced during SSF was 44.25 g/L. Nonetheless, pre-hydrolysis simultaneous saccharification and fermentation gave 31.22 g/L (CP). All results suggested that optimized two step pretreatment produced efficient ethanol.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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