Reference: Zhang S, et al. (2021) Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast. Methods Mol Biol 2196:235-244

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Abstract


Live-cell imaging is widely used by researchers to study cellular dynamics and obtain a deep understanding of cell biological processes. Keeping cells in the proper growing environment and immobilizing the cells are essential for the imaging of live yeast cells. Here we describe a protocol for monitoring cytoophidia in Saccharomyces cerevisiae and Schizosaccharomyces pombe using inverted confocal fluorescence microscopy. This protocol includes yeast culture, sample preparation, fluorescence imaging, and data analysis.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Zhang S, Li H, Liu JL