Reference: Zhu J and Xie Z (2021)
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Abstract
Macroautophagy, by its very nature, is a protein trafficking process. Cargos are transported and processed. Atg proteins come and go. In this chapter, we present three assays to monitor these dynamic events: a non-radioactive pulse-chase labeling assay to monitor the transport of prApe1 and two fluorescent microscopy-based assays to assess the trafficking of Atg8 and Atg9.
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Journal Article
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Zhu J,
Xie Z
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- ATG1 | APE1 | ATG9 | ATG8
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