The aim of this work was to bioconvert all sugars in BSG into ethanol using a process scheme that includes the enzymatic hydrolysis of the whole slurry resulting from the pretreatment of BSG with phosphoric and sulfuric acid using previously optimised conditions, followed by the co-fermentation of the mixed sugars. More than 90% of the sugars in raw BSG were recovered in the pretreatment and the subsequent enzymatic hydrolysis of the whole slurry. The co-fermentation of the enzymatic hydrolysates with Escherichia coli was then compared with that the co-culture of Scheffersomyces stipitis and Saccharomyces cerevisiae, which resulted in lower ethanol production. The co-fermentation strategy with a single microorganism (E. coli) when BSG was pretreated with phosphoric acid resulted into the highest ethanol concentration, 39 g/L, which means that 222 L of ethanol can be obtained from a ton of BSG without detoxification requirements.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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