We have cloned the met2 gene from Ascobolus immersus by heterologous hybridization with the MET2 gene of Saccharomyces cerevisiae. This gene codes for the homoserine O-transacetylase, one of the methionine biosynthetic enzymes. The complete nucleotide sequence of a 2910-bp DNA fragment carrying the met2 gene has been determined. The gene contains a 165-bp intron which is similar in structure to other fungal introns. The deduced amino acid (aa) sequence (518 aa residues; Mr of 57726) shows three domains with a significant level of homology with the corresponding yeast protein. Northern-blot analysis reveals at least two transcripts (2.4 and 2.1 kb) probably due to transcription termination heterogeneity, as suggested by S1-mapping experiments. Polymorphism has been observed in the met2 gene flanking regions of Ascobolus strains from two different stocks.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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