Reference: Andrews BJ and Herskowitz I (1989) Identification of a DNA binding factor involved in cell-cycle control of the yeast HO gene. Cell 57(1):21-9

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Abstract


Transcription of the HO gene is controlled by at least eleven trans-acting regulators, which are responsible for limiting expression to mother cells and to one period in the cell cycle. A subset of these regulators and a repeated element (the cell-cycle box, CCB) are required for expression of HO during late G1. We describe gel retardation and footprinting experiments that identify a factor, CCBF (cell-cycle box factor), that binds to multiple cell-cycle box elements. We show that the SWI4 and SWI6 genes are required for formation of the CCBF-promoter complex in vitro, either as components of CCBF or as modulators of CCBF activity. The other SWI genes (SWI1, 2, 3, and 5) are not required. We also show that SWI4 and SWI6 are the only SWI genes required for expression in vivo from the CCB sequences. These observations indicate that the CCBF is responsible for cell-cycle regulation of HO and lead to the view that two of the SWI genes, SWI4 and SWI6, are specifically involved in this regulatory event.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Andrews BJ, Herskowitz I
Primary Lit For
SWI6 | SWI4
Additional Lit For
HO | SWI5 | SWI1

Gene Ontology Annotations 5 entries for 2 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
SWI4involved inpositive regulation of transcription by RNA polymerase IIpart of G1/S transition of mitotic cell cycleIMPSGD2023-09-08
SWI4involved inG1/S transition of mitotic cell cycleIMPSGD2023-09-08
SWI4enablesDNA-binding transcription activator activity, RNA polymerase II-specificIMPSGD2019-09-20
SWI6involved inpositive regulation of transcription by RNA polymerase IIpart of G1/S transition of mitotic cell cycleIMPSGD2023-09-08
SWI6involved inG1/S transition of mitotic cell cycleIMPSGD2023-09-08
Showing 1 to 5 of 5 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 1 entry for 2 genes

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InteractorInteractorAssayAnnotationActionModification
SWI4SWI6Reconstituted Complexmanually curatedBait-HitNo Modification
Showing 1 to 1 of 1 entries