Transcription of the HO gene is controlled by at least eleven trans-acting regulators, which are responsible for limiting expression to mother cells and to one period in the cell cycle. A subset of these regulators and a repeated element (the cell-cycle box, CCB) are required for expression of HO during late G1. We describe gel retardation and footprinting experiments that identify a factor, CCBF (cell-cycle box factor), that binds to multiple cell-cycle box elements. We show that the SWI4 and SWI6 genes are required for formation of the CCBF-promoter complex in vitro, either as components of CCBF or as modulators of CCBF activity. The other SWI genes (SWI1, 2, 3, and 5) are not required. We also show that SWI4 and SWI6 are the only SWI genes required for expression in vivo from the CCB sequences. These observations indicate that the CCBF is responsible for cell-cycle regulation of HO and lead to the view that two of the SWI genes, SWI4 and SWI6, are specifically involved in this regulatory event.
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Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
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SWI4 | involved in | positive regulation of transcription by RNA polymerase II | part of G1/S transition of mitotic cell cycle | IMP | SGD | 2023-09-08 |
SWI4 | involved in | G1/S transition of mitotic cell cycle | IMP | SGD | 2023-09-08 | |
SWI4 | enables | DNA-binding transcription activator activity, RNA polymerase II-specific | IMP | SGD | 2019-09-20 | |
SWI6 | involved in | positive regulation of transcription by RNA polymerase II | part of G1/S transition of mitotic cell cycle | IMP | SGD | 2023-09-08 |
SWI6 | involved in | G1/S transition of mitotic cell cycle | IMP | SGD | 2023-09-08 |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.