Simultaneous inhibition of oxidative phosphorylation by rho- mutation and adenine nucleotide exchange by op1 mutation or bongkrekic acid results in intramitochondrial energy depletion and cessation of growth in yeast. Effect of energy depletion of mitochondria on mitochondrial biogenesis was studied in intact yeast cells. Immunoblot analysis revealed an overall decrease in cellular content of two mitochondrial proteins - ADP/ATP translocase and beta subunit of mitochondrial ATPase - together with their lower ability to reach the proper intramitochondrial compartment. Both effects indicate disturbed biogenesis of energy depleted mitochondria. Quantitative differences in growth abilities and mitochondrial damage observed in two studied systems - op1 rho- double mutants and rho- cells treated with bongkrekic acid - can be explained by different degree of intramitochondrial energy depletion due to leakiness of op1 mutation in op1 rho- cells.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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