We present a new tomographic phase microscopy (TPM) approach that allows capturing the three-dimensional refractive index structure of single cells in suspension without labeling, using 180° rotation of the cells. This is obtained by integrating an external off-axis interferometer for wide-field wave front acquisition with holographic optical tweezers (HOTs) for trapping and micro-rotation of the suspended cells. In contrast to existing TPM approaches for cell imaging, our approach does not require anchoring the sample to a rotating stage, nor is it limited in angular range as is the illumination rotation approach. Thus, it allows noninvasive TPM of suspended live cells in a wide angular range. The proposed technique is experimentally demonstrated by capturing the three-dimensional refractive index map of yeast cells, while collecting interferometric projections at an angular range of 180° with 5° steps. The interferometric projections are processed by both the filtered back-projection method and the diffraction theory method. The experimental system is integrated with a spinning disk confocal fluorescent microscope for validation of the label-free TPM results.

• PMID: 25872098
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Journal Article | Research Support, Non-U.S. Gov't

Authors

Habaza M, Gilboa B, Roichman Y, Shaked NT

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