Mutants of the yeast Saccharomyces cerevisiae carrying ribosomal suppressor mutations in either sup1 or sup2 genes express a higher sensitivity to paromomycin - aminoglycoside antibiotic known to induce translational errors in eukaryotes. Paromomycin also induces a phenotypic suppression of all three types of nonsense mutations (ochre, amber and opal), missense mutations and frame-shift mutations. The influence of paromomycin on the activity of ribosomal suppressors has at least two aspects: (1) the drug increases translational ambiguity in sup1 and sup2 mutants in vitro and (2) it induces the alteration (extension or restriction) of sup 1 or sup2 suppression spectra in vivo. A modification of selectivity of the mutant ribosomes towards different tRNAs in the presence of paromomycin is proposed.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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