The activity of a yeast mitochondrial endonuclease extracted from mutants (sas1) with increased sensitivity to petite-inducing treatments was compared to that from wild-type cells. The specificity of the endonuclease was altered in haploids carrying a single mutant nuclear gene that conferred increased sensitivity to petite induction by ultraviolet light, by growth at an elevated temperature and by growth in the presence of aminopterin and sulfanilamide. At high ionic strengths the endonuclease from the mutants digested double stranded DNA much faster than did that from the wild-type strain. Also the mutant enzyme was less selective for poly(dA) poly(dU) in comparison to poly(dA) · poly(dT); it had less preference for reduced hydrogen bond strength between the strands of double stranded DNA. Results indicate that this endonuclease, as a degrading enzyme, is involved in the initial step in repair of damaged mitochondrial DNA.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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