Stojkovič G and Znidaršič-Plazl P (2010)

Abstract

Saccharomyces cerevisiae was successfully immobilized on the inner wall surface of channels of submillimeter diameter, which can be further used for the development of a highly productive continuous biotransformation process within a microfluidic device. Covalent bonding by means of 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde was used for immobilization of cells to microchannels made of glass, polystyrene (PS), polytetrafluoroethylene (PTFE), perfluoroalkoxy (PFA) and fluorinated ethylene propylene (FEP). All tested materials were successfully functionalized with H2SO4 to promote silanization. The effect of reaction time with acid on immobilization efficiency was studied for polymer materials. This is the first report on cell immobilization onto PTFE, FEP and PFA surface, which enables to develop a microfluidic device with surface bound biocatalyst from low cost and disposable materials.

PMID: 24061666
PubMed
PubTator

Download Citation (.nbib)

Reference Type: Journal Article
Authors: Stojkovič G, Znidaršič-Plazl P
Primary Lit For
Additional Lit For
Review For

Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, or SPELL.

Interaction Annotations