Reference: Benders GA (2012) Cloning whole bacterial genomes in yeast. Methods Mol Biol 852:165-80

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Abstract


Many bacterial and archaeal genomes are of a similar size to molecules that have been cloned in the yeast Saccharomyces cerevisiae and thus might be clonable as single, circular episomes in this host. Yeast offers a variety of efficient tools for the manipulation and study of cloned DNA. One strategy to clone a genome in yeast is to cotransform yeast spheroplasts with the genome of interest and a linear yeast vector whose termini are homologous to a spot in the genome. Clones are selected on auxotrophic medium and then screened for completeness and size; they may also be sequenced.

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Journal Article | Research Support, Non-U.S. Gov't
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Benders GA
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