Reference: Dai J and Boeke JD (2012) Strain construction and screening methods for a yeast histone H3/H4 mutant library. Methods Mol Biol 833:1-14

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Abstract


A mutant library consisting of hundreds of designed point and deletion mutants in the genes encoding Saccharomyces cerevisiae histones H3 and H4 is described. Incorporation of this library into a suitably engineered yeast strain (e.g., bearing a reporter of interest), and the validation of individual library members is described in detail.

Reference Type
Journal Article
Authors
Dai J, Boeke JD
Additional Lit For
HHF1 | HHT1 | HHF2 | HHT2