Reference: Aleksic J and Russell S (2009) ChIPing away at the genome: the new frontier travel guide. Mol Biosyst 5(12):1421-8

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Abstract


Chromatin immunoprecipitation (ChIP) is a powerful technique for obtaining in vivo data on protein-DNA binding, providing an invaluable tool for elucidating gene regulation at a molecular level. Combined with high-throughput methods such as microarrays (ChIP-array) and second generation sequencing (ChIP-seq), the technique is now commonly used for answering questions about protein binding on a genome-wide level. This review focuses on the use of microarrays and sequencing for ChIP studies, provides a critical comparison of the currently used platforms and an overview of the computational methods available, and offers recommendations for optimal use of the techniques in a research context.

Reference Type
Journal Article | Review
Authors
Aleksic J, Russell S
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Gene Disease Ontology Term Qualifier Evidence Method Source Assigned On Reference

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

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Gene Species Gene ID Strain background Direction Details Source Reference