Reference: Olivas WM (2008) Identification of changes in gene expression by quantitation of mRNA levels. Methods Mol Biol 419:243-58

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Abstract


Sequence elements within mRNA-untranslated regions and their binding partners are key controllers of mRNA stability. Changes in mRNA stability can often be detected by changes in steady-state mRNA abundance, or a more careful analysis of mRNA half-lives can be performed following transcriptional repression. This chapter presents methods to isolate RNA from both yeast and mammalian cells for either steady-state or half-life analyses. In addition, two reliable methods to quantitate mRNA levels, northern blot analysis and real-time PCR, are outlined and compared.

Reference Type
Journal Article | Research Support, N.I.H., Extramural
Authors
Olivas WM