Reference: Gai SA and Wittrup KD (2007) Yeast surface display for protein engineering and characterization. Curr Opin Struct Biol 17(4):467-73

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Abstract


Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein-protein interactions, represents a significant recent advance.

Reference Type
Journal Article | Research Support, N.I.H., Extramural | Research Support, U.S. Gov't, Non-P.H.S. | Review
Authors
Gai SA, Wittrup KD
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Gene Species Gene ID Strain background Direction Details Source Reference