Reference: Pasha C, et al. (2007) Lantana camara for fuel ethanol production using thermotolerant yeast. Lett Appl Microbiol 44(6):666-72

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Abstract


Aim: Evaluation of Lantana camara's use as feedstock for fuel ethanol production.

Methods and results: Lantana camara plant material was hydrolysed with 1% sulfuric acid for 18 h at room temperature, followed by heat treatment of 121 degrees C for 20 min. Hemicellulosic hydrolyzate was separated and used for detoxification by ethyl acetate and overliming. Cellulosic fraction was hydrolysed with Aspergillus niger crude cellulase enzyme for 18 h at 55 degrees C. Using 15% (dw/v) substrate 73 g l(-1) total reducing sugars were obtained to give 78.7% hydrolysis of carbohydrate content. Acid and enzyme hydrolyzates were mixed equally and used for fermentation with thermotolerant Saccharomyces cerevisiae (VS(3)). Yeast fermented L. camara hydrolyzate well with a fermentation efficiency of 83.7% to give an ethanol yield of 0.431 +/- 0.018 g ethanol pre g sugar and productivity of 0.5 +/- 0.021 g l(-1) h(-1).

Conclusions: Even though inhibitors were present in L. camara hydrolyzate, maximum sugars were utilized by thermotolerant yeast.

Significance and impact of the study: Use of L. camara for fuel ethanol production with improved strains and detoxification can be recommended.

Reference Type
Evaluation Study | Journal Article | Research Support, Non-U.S. Gov't
Authors
Pasha C, Nagavalli M, Rao LV
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