Since the cold denaturation of most proteins occurs well below 0 degrees C, full access to the cold denatured state is normally limited by water freezing. To circumvent this difficulty the temperature of cold denaturation is normally raised, destabilizing the protein by point mutations and using chemical or physical denaturation. The main drawback of these approaches is that it is not generally possible to extrapolate the results to physiological conditions. Following a different approach, we looked for a protein whose cold denaturation could be studied in a normal buffer at physiological pH without the need of destabilization, in a temperature range accessible to several techniques. Here we describe the cold and heat denaturation of yeast frataxin (Yfh1). Both NMR and CD data were fitted by the same equation, showing that the collapse of the hydrophobic core is exactly paralleled by a decrease of the secondary structure content.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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