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Reference: Siniossoglou S (2005) Affinity purification of Ypt6 effectors and identification of TMF/ARA160 as a Rab6 interactor. Methods Enzymol 403:599-607

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Abstract

Rab/Ypt GTPases are key regulators of intracellular traffic in eukaryotic cells. One important function of Rab/Ypts is the nucleotide-dependent recruitment of downstream effector molecules onto the membrane of organelles. In budding yeast Ypt6 is required for recycling of membrane proteins from endosomes back to the Golgi. A biochemical approach based on the affinity purification of Ypt6:GTP-interacting proteins from yeast cytosol led to the identification of two conserved Ypt6 effectors, the tetrameric VFT complex and Sgm1. The mammalian homolog of Sgm1, TMF/ARA160, contains a short conserved coiled-coil motif that is sufficient for the binding to the three mammalian orthologs of Ypt6, Rab6A, Rab6A', and Rab6B.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Siniossoglou S
Primary Lit For
VPS54 | VPS53 | SGM1 | YPT6 | VPS52

Interaction Annotations

Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 8 entries for 6 genes

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InteractorInteractorAssayAnnotationActionModification
SGM1YPT6Affinity Capture-MSmanually curatedHit-BaitNo Modification
SGM1YPT6Affinity Capture-Westernmanually curatedHit-BaitNo Modification
VPS52YPT6Affinity Capture-MSmanually curatedHit-BaitNo Modification
VPS52YPT6Affinity Capture-Westernmanually curatedHit-BaitNo Modification
VPS53YPT6Affinity Capture-MSmanually curatedHit-BaitNo Modification
VPS53YPT6Affinity Capture-Westernmanually curatedHit-BaitNo Modification
VPS54YPT6Affinity Capture-Westernmanually curatedHit-BaitNo Modification
YPT31VPS54Affinity Capture-Westernmanually curatedBait-HitNo Modification
Showing 1 to 8 of 8 entries