Cloning and sequencing of the pho2 gene which codes for a specific p-nitrophenylphosphatase from Schizosaccharomyces pombe is described. The gene has an open contiguous reading frame of 269 amino acids corresponding to a protein with a molecular mass of 29.5 kDa and a calculated pI of 6.6. The sequence reveals four regions that share significant sequence similarity with the corresponding gene PHO13 of Saccharomyces cerevisiae. Purification of the enzyme to apparent homogeneity is reported. The amino acid composition of the purified protein matches well the values predicted from the nucleotide sequence. On SDS/polyacrylamide gels, the enzyme runs as a protein with a molecular mass of 33 kDa, and by Sephadex chromatography under nondenaturing conditions as 70 kDa. This indicates that the enzyme is a homodimer in its native form. The enzyme is not glycosylated. Its activity is stimulated by Mg2+ and inhibited by Zn2+. The available data on p-nitrophenylphosphatase do not give any clues to its biological role and its physiological substrates.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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