Reference: Bigay J and Antonny B (2005) Real-time assays for the assembly-disassembly cycle of COP coats on liposomes of defined size. Methods Enzymol 404:95-107

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Abstract


The assembly-disassembly cycle of COPI and COPII coats is controlled by the GTPase cycle of the small G proteins Arf1 and Sar. We describe here two spectroscopic assays that enable real-time studies of some elementary steps of coat assembly and disassembly on artificial liposomes of defined composition and curvature. A flotation assay to assess the effect of membrane curvature on protein adsorption to liposomes is also presented.

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Journal Article
Authors
Bigay J, Antonny B
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