Reference: Ptak C, et al. (2005)
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Abstract
A prerequisite for structure/function studies on the ubiquitin-conjugating enzymes (Ubc) Cdc34 and Ubc13.Mms2 has been the ability to express and purify recombinant derivatives of each. This chapter describes the methods used in the expression and purification of these proteins from Escherichia coli, including variations of these protocols used to generate (35)S, (15)N, (13)C/(15)N, and seleno-L-methionine derivatives. Assays used to measure the Ub thiolester and Ub conjugation activities of these Ubcs are also described.
- Reference Type
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Journal Article
- Authors
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Ptak C,
Varelas X,
Moraes T,
McKenna S,
Ellison MJ
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