Reference: Tackett AJ, et al. (2005) I-DIRT, a general method for distinguishing between specific and nonspecific protein interactions. J Proteome Res 4(5):1752-6

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Abstract


Isolation of protein complexes via affinity-tagged proteins provides a powerful tool for studying biological systems, but the technique is often compromised by co-enrichment of nonspecifically interacting proteins. We describe a new technique (I-DIRT) that distinguishes contaminants from bona fide interactors in immunopurifications, overcoming this most challenging problem in defining protein complexes. I-DIRT will be of broad value for studying protein complexes in biological systems that can be metabolically labeled.

Reference Type
Journal Article | Research Support, N.I.H., Extramural | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Tackett AJ, DeGrasse JA, Sekedat MD, Oeffinger M, Rout MP, Chait BT
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Interaction Annotations


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