The plasma membrane is dynamic, with both its lipid and protein composition changing to facilitate adaptation to the ambient conditions. Biochemical activities to pre-existing proteins will also change. To monitor these variations, the cell membrane must be isolated. Moreover, the preparations must be free of contamination from the variety of other membranes in the cell, principally those associated with the golgi, endoplasmic reticulum (ER), the nucleus, and the vacuole. We describe a method for isolating plasma membranes that avoids incubation with enzymes that degrade the cell wall, thereby avoiding physiological changes that may lead to alteration in profile and activity of membrane proteins as well as avoiding changes that may alter lipid composition. We have used this method to show that, in response to heat shock, the plasma membrane acquires a novel heat-shock protein (HSP) and displays a decline in the levels of the abundant H+ translocating ATPase.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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