Yeast mutants lacking meiotic proteins Zip1, Zip2, Zip3, Mer3, and/or Msh5 (ZMMs) were analyzed for recombination, synaptonemal complex (SC), and meiotic progression. At 33 degrees C, recombination-initiating double-strand breaks (DSBs) and noncrossover products (NCRs) form normally while formation of single-end invasion strand exchange intermediates (SEIs), double Holliday junctions, crossover products (CRs), and SC are coordinately defective. Thus, during wild-type meiosis, recombinational interactions are differentiated into CR and NCR types very early, prior to onset of stable strand exchange and independent of SC. By implication, crossover interference does not require SC formation. We suggest that SC formation may require interference. Subsequently, CR-designated DSBs undergo a tightly coupled, ZMM-promoted transition that yields SEI-containing recombination complexes embedded in patches of SC. zmm mutant phenotypes differ strikingly at 33 degrees C and 23 degrees C, implicating higher temperature as a positive effector of recombination and identifying a checkpoint that monitors local CR-specific events, not SC formation, at late leptotene.
Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.
| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| MSH5 | involved in | reciprocal meiotic recombination | IMP | SGD | 2013-08-07 | |
| ZIP1 | involved in | reciprocal meiotic recombination | IMP | SGD | 2013-08-07 | |
| ZIP2 | involved in | reciprocal meiotic recombination | IMP | SGD | 2013-08-07 | |
| HFM1 | involved in | reciprocal meiotic recombination | IMP | SGD | 2013-08-07 | |
| CST9 | involved in | reciprocal meiotic recombination | IMP | SGD | 2013-08-07 |
Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| HFM1 | protein/peptide distribution: abnormal Reporter: Zip1p | classical genetics | null Allele: hfm1-Δ | SK1 | ||
| MSH5 | protein/peptide distribution: abnormal Reporter: Zip1p | classical genetics | null Allele: msh5-Δ | SK1 | ||
| ZIP2 | protein/peptide distribution: abnormal Reporter: Zip1p | classical genetics | null Allele: zip2-Δ | SK1 | ||
| CST9 | protein/peptide distribution: abnormal Reporter: Zip1p | classical genetics | null Allele: cst9-Δ | SK1 |