Systems catalyzing the reduction of sulfate in vitro have been described for Aspergillus (l), yeast (2-4), and Desulfovibrio (5, 6). Sulfate reduction and the associated pathways leading to cysteine have recently been reviewed (7). In the present study, and in previous brief reports (4, 8, 9), it is shown that sulfate activation (10, II), reactions 1 and 2, precedes sulfate reduction. ATP + SO4= <-> APS1 + PPi (1), APS + ATP <-> PAPS + ADP (2). The reduction of 3'-phosphoadenosine-5'-phosphosulfate or "active sulfate" is shown to involve two heat-labile protein fractions, designated Enzymes A and B, and a nondialyzable, heat-stable protein, Fraction C (2). The over-all sulfate-reducing system is considered in this paper, whereas a more detailed study of Enzyme A and Fraction C is the subject of a separate report (12).

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Journal Article

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WILSON LG, ASAHI T, BANDURSKI RS

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