Removal of thioctic acid from enzyme proteins may be accomplished by treatment of crude tissue or cell extracts with adsorption alumina (1). This procedure lowers the thioctic acid content of alpha-keto acid oxidases in cell-free extracts of Tetrahymena (2). King et al. (3) have also used the procedure to reduce the thioctic acid content of extracts of Acetobacter suboxydans. The effectiveness of the procedure is dependent (4) upon the presence in crude extracts of an enzyme which releases protein-bound thioctic acid; the liberated cofactor is then adsorbed on the alumina. Crude preparations of this enzyme have been obtained by a single ammonium sulfate fractionation of pigeon liver acetone powder (1, 4). Reed (5) recently reported a "lipoyl-x hydrolase" in Streptococcus faecalis; this is apparently the enzyme responsible for that decrease in thioctic acid content of crude extracts of this organism which is achieved by alumina treatment (1). The present report describes a procedure for purification of an enzyme from baker's yeast which liberates protein-bound thioctic acid.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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