In Saccharomyces cerevisiae, alpha 2/Mcm1p represses a-cell specific genes. Previous studies indicated that repression by alpha 2/Mcm1p is associated with organization of positioned nucleosomes in promoters of a-cell specific genes such as STE6 and BAR1. Here, we examined the role of nucleosome positioning in repression of STE6-lacZ by alpha 2/Mcm1p. We showed that insertion of an A34 sequence, which is shown to disrupt an array of positioned nucleosomes, affects STE6-lacZ expression. Furthermore, artificial recruitment of TBP is facilitated by the insertion of A34 to derepress STE6-lacZ expression. These findings support the model that nucleosome positioning plays a regulatory role in repression by alpha 2/Mcm1p.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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