A gene encoding Delta9 fatty acid desaturase, which catalyzes the first reaction of fatty acid desaturation, has been cloned from Saccharomyces kluyveri, a species capable of producing polyunsaturated fatty acids (poly-UFA). The open reading frame of this gene (named Sk-OLE1) consists of 1443 bp, encoding 480 amino acids. The deduced Sk-OLE1 protein shared 63-74% identity with those from other yeasts. Expression of the Sk-OLE1 gene in Saccharomyces cerevisiae complemented genetic disruption of the S. cerevisiae OLE1 gene (Sc-OLE1). Fatty acid analysis showed that the ratio of palmitoleic acid to oleic acid in the Sk-OLE1-expressing S. cerevisiae strain was lower than that of the S. cerevisiae strain expressing the Sc-OLE1 gene. The promoter region of the Sk-OLE1 gene was shorter than that of the Sc-OLE1 gene and appeared to lack the fatty acid-regulated sequences present in the Sc-OLE1 promoter region. Analysis of transcriptional expression revealed that the Sk-OLE1 gene was not actually repressed in either S. kluyveri or S. cerevisiae by exogenous UFAs. The DDBJ accession number for the sequence reported in this paper is AB071696.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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