A novel process for the ATP production employing free yeast catalysis and coupling a hollow fiber module ultrafiltration separation was studied. The experimental results of biocatalyst half-life and operating stability for ultrafiltration showed that the enzymes released from free yeast cell could be cut-off efficiently from the product output stream by hollow fiber membrane. The intercepted percentage of ADH and HK reached more than 95% during the steady running. The efficiency of enzymes utilized was 2.0-2.5 fold as much as that of batch reaction. And the time of yeast cells used repeatedly for ATP synthesis reached 2.5-3.0 batches. Under 0.1 MPa, the ultrafiltrating speed of hollow fiber membrane could keep on a stabilizing value for 11 batches continuously. The continuous operation mode of ATP synthesis simultaneous product ultrafiltration separation could be maintained in higher conversion rate for 5.0 h when the whole system fell in a pseudo-stat where the dilution rate was equal to 0.25 h-1.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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