Aims: The objectives of this study were to investigate the potential application of a low-amperage direct electric current as a non-thermal process for inactivation of Saccharomyces cerevisiae.
Methods and results: Electric current was generated using a direct current power supply connected to a traditional electrochemical cell with two platinum electrodes immersed in conducting solution containing a population of S. cerevisiae. This treatment provoked inactivation of the yeast cells. The microbial destruction illustrated by D-values calculated from survival curves was shown to be proportional to the current amperage (i) (D varies from 1547 min to 140 min when i varies from 0.1 to 1 A, respectively). The efficacy of the treatment was shown to be better at pH < 7. Statistical analysis showed no significant effect (P > 0.05) of ionic strength on yeast lethality induced by electrolysis.
Conclusions: The lethal effect of the electric treatment on S. cerevisiae in phosphate buffer was shown to be due to neither ohmic heating nor toxic hydrogen peroxide. A synergistic effect of temperature and electrolysis was observed when the temperature became lethal for the yeast.
Significance and impact of the study: The method described for yeast lethality induced by electrolysis has potential for soft sterilization, particularly when combined with the synergistic effect of moderate heat.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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