Reference: Rozenfeld S and Thuriaux P (2001) A genetic look at the active site of RNA polymerase III. EMBO Rep 2(7):598-603

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Abstract


rpc160-112, a mutant of the RNA polymerase III active site, is corrected in vivo by six second-site mutants obtained by random mutagenesis. These mutants introduce single-site amino acid replacements at the two large subunits of the enzyme. The mutated motifs are conserved in RNA polymerases I and II and, for some of them, in the bacterial enzyme, thus delineating key elements of the active site in eukaryotic RNA polymerases.

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Journal Article | Research Support, Non-U.S. Gov't
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Rozenfeld S, Thuriaux P
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