Aims: Saccharomyces cerevisiae was used for studying the physiological effects of hydrostatic pressure.
Methods and results: The effects of hydrostatic pressure on the ultrastructure of wild-type and trehalose-6-phosphate synthase (tps1) mutant cells were investigated by transmission electron microscopy. Pressure induced several morphological changes in wild-type and tps1 cells, the latter showing greater structural alterations. When the cells were submitted to a preheat treatment they both acquired resistance to the pressure treatment.
Conclusion: As the tps1 mutant was 1000-fold more barosensitive than its parental strain, it showed greater structural alterations compared with the wild-type. Microscopic images of the yeast cells suggested that hydrostatic pressure induced changes in the cytoskeleton and therefore, on the cell wall and in the dynamics of the organelles.
Significance and impact of the study: This work presents the effects of hydrostatic pressure on the morphology of yeast cells and confirms the importance of several different factors in the protection of cells against stress.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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