Reference: Melo JS and D'Souza SF (2000) A simple approach for the simultaneous isolation and immobilization of invertase using crude extracts of yeast and Jack bean meal. J Biochem Biophys Methods 42(3):133-5

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Abstract


Crude cell-free extract of yeast cells was mixed with sufficient amount of Jack bean meal extract so as to precipitate all the invertase. The precipitate was then cross-linked using 2% glutaraldehyde retaining over 60% of the activity. The immobilized invertase could be reused for over ten batches without loss in activity.

Reference Type
Journal Article
Authors
Melo JS, D'Souza SF