Metabolism of 13C labeled substrates viz. glucose and pyruvate in S. cerevisiae has been studied by 13C Nuclear Magnetic Resonance Spectroscopy. C3-Pyruvate, alanine and lactate, and C2-acetate are produced from [1-13C]glucose. The pyruvate, entering TCA cycle, leads to preferential labeling of C2-glutamate. [2-13C]Glucose results in labeling of C2-pyruvate, alanine and lactate. Some C3-pyruvate is also produced, indicating the routing of the label from glucose through pentose phosphate pathway (PPP). In TCA cycle the C2-pyruvate preferentially labels the C3-glutamate. The NMR spectra, obtained with [2-13C]pyruvate as substrate, confirm the above observations. These results suggest that the intermediates of TCA cycle are transferred from one enzyme active site to another in a manner that allows only restricted rotation of the intermediates. That is, the intermediates are partially channeled.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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