REB1 / YBR049C Overview


Standard Name
REB1 1
Systematic Name
YBR049C
SGD ID
SGD:S000000253
Aliases
GRF2 3 , EBF1 4
Feature Type
ORF , Verified
Description
RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; Reb1p bound to DNA acts to block RNA polymerase II readthrough transcription 1 2
Name Description
RNA polymerase I Enhancer Binding protein 1
Paralog
NSI1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
810
Mol. Weight (Da)
91825.5
Isoelectric Point
4.76
Median Abundance (molecules/cell)
5582 +/- 1829
Half-life (hr)
7.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all REB1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Sequence-specific DNA binding protein in the nucleus involved in regulation of transcription by RNA polymerase II, and in the termination of transcription by RNA polymerase I

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene in strain S288C, in which conditional mutants are heat-sensitive, and reduced function mutants are sensitive to microtubule-destabilising agent benomyl; non-essential gene in strain Sigma1278b, in which null mutants are impaired in filamentous growth
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The reb1 null mutant is inviable; the null mutant of paralog nsi1 is viable; the reb1 nsi1 double mutant has not been annotated for phenotype.

669 total interactions for 641 unique genes

Physical Interactions

  • Affinity Capture-MS: 55
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 1
  • Co-localization: 1
  • Co-purification: 1
  • FRET: 2
  • PCA: 3
  • Protein-RNA: 3
  • Proximity Label-MS: 1

Genetic Interactions

  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 334
  • Positive Genetic: 254
  • Synthetic Growth Defect: 3
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
REB1 encodes an essential myb-family transcription factor initially implicated in the regulation of ribosomal RNA transcription. The Reb1p consensus binding site (5'-CGGGTRR-3'), however, is also found in the promoters of a large number of genes transcribed by RNA Polymerase II, as well as in centromeres and in subtelomeric regions. Further studies have suggested that Reb1p may indeed function as a weak transcriptional activator of a variety of protein-coding genes throughout the genome, including ACT1, FAS1 and FAS2, CLN2. Multiple Reb1p binding sites are located within the subtelomeric regions, where they appear to act as anti-silencing barriers that prevent the spreading of silenced chromatin. More recently, Reb1p has been identified as one of several factors, including Abf1p, Rsc3p, Rap1p, Mcm1p, Tbf1p and Cep3p, whose binding to the promoter region prevents nucleosome formation in the vicinity, thus generating a nucleosome-free region around the promoter and facilitating the process of transcription initiation.
Regulators
10
Targets
1001
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
60
Additional
134
Reviews
31

Resources