DED1 / YOR204W Overview


Standard Name
DED1 1
Systematic Name
YOR204W
SGD ID
SGD:S000005730
Aliases
SPP81 10
Feature Type
ORF , Verified
Description
ATP-dependent DEAD-box RNA helicase with strand-annealing activity; promotes eIF4F-dependent 48S translation preinitiation complex (PIC) assembly, stimulating recruitment of mRNAs with long, structured 5’-UTRs; cooperates with Dbp1p in PIC attachment and scanning; ATPase activity stimulated by mRNA cap-associated factor binding; directly binds eIF4G; role in spliceosomal complex disassembly; mutation in human homolog DBY associated with male infertility; human homolog DDX3X complements the null 2 3 4 5 6 7 8 9
Name Description
Defines Essential Domain 1
Paralog
DBP1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
604
Mol. Weight (Da)
65554.7
Isoelectric Point
7.98
Median Abundance (molecules/cell)
25034 +/- 5043
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Bifunctional enzyme with both RNA strand-annealing and ATP-dependent RNA helicase activities; translation initiation factor involved in assembly of the translational preinitiation complex and disassembly of the spliceosomal complex; localizes to the nucleus, cytoplasm, and cytoplasmic stress granule

View computational annotations

Molecular Function

Manually Curated
High-Throughput

Cellular Component

Manually Curated
High-Throughput
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene; conditional alleles confer cold sensitivity and slow growth even at permissive temperature; in a large-scale study, mutant exhibits MMS sensitivity
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The ded1 null mutant is inviable; the null mutant of paralog dbp1 is viable; the ded1 dbp1 double mutant shows decreased growth.

781 total interactions for 606 unique genes

Physical Interactions

  • Affinity Capture-MS: 167
  • Affinity Capture-RNA: 9
  • Affinity Capture-Western: 17
  • Biochemical Activity: 3
  • Co-fractionation: 1
  • Co-purification: 2
  • PCA: 12
  • Proximity Label-MS: 1
  • Reconstituted Complex: 16
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Growth Defect: 3
  • Dosage Lethality: 1
  • Dosage Rescue: 15
  • Negative Genetic: 359
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 5
  • Positive Genetic: 145
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 10
  • Synthetic Rescue: 5
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
12
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
60
Additional
68
Reviews
22

Resources