RPT1 / YKL145W Overview

Standard Name
RPT1 1
Systematic Name
CIM5 7 , YTA3 8
Feature Type
ORF , Verified
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance 2 3 4 5 6
Name Description
Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

RPT1 is located on the left arm of chromosome XI between AVT3 vacuolar transporter and RPC25 RNA polymerase III subunit, dubious ORF YKL145W-A is located completely within RPT1 on the same strand; coding sequence is 1404 nucleotides long with 7 silent SNPs
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Rpt1p is 467 amino acids long, somewhat longer-lived, and moderate in abundance; contains AAA ATPase core domain and AAA ATPase lid domain; acetylated on 3 lysines, phosphorylated on 7 serines, ubiquitinylated on 7 lysines
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
12952 +/- 3058
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all RPT1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Putative ATPase involved in proteasome regulatory particle assembly, ubiquitin-dependent protein degradation and positive regulation of RNA polymerase II transcriptional preinitiation complex assembly; subunit of 19S regulatory particle

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Essential gene; cim5-1 ts allele causes arrest with large buds at restrictive temperature, accumulation of degradation intermediates, and increased resistance to 4-NQO; rpt1-K256S mutation causes shorter replicative lifespan, sensitivity to canavanine, and decreased budding index; in large-scale studies, repression causes decreased competitive fitness; diploid heterozygous null mutant is haploinsufficient
Disease Details


Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.

Yeast RPT1 is homologous to human PSMC2, and has been used to study cancer

Manually Curated

Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Rpt1p interacts physically with proteins involved in protein catabolism; RPT1 interacts genetically with genes involved in mitosis

1160 total interactions for 668 unique genes

Physical Interactions

  • Affinity Capture-MS: 246
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 123
  • Co-crystal Structure: 3
  • Co-fractionation: 8
  • Co-localization: 1
  • Co-purification: 44
  • PCA: 7
  • Reconstituted Complex: 41
  • Two-hybrid: 4

Genetic Interactions

  • Dosage Rescue: 2
  • Negative Genetic: 531
  • Phenotypic Enhancement: 2
  • Positive Genetic: 134
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

RPT1 promoter is bound by Reb1p and Rpn4p; promoter bound by Xbp1p in response to heat; transcription of RPT1 is regulated by Rpn4p, regulated by Cad1p under oxidative stress, downregulated by Ixr1p in under hypoxia
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.