APQ12 / YIL040W Overview
- Standard Name
- APQ12 1
- Systematic Name
- YIL040W
- SGD ID
- SGD:S000001302
- Feature Type
- ORF , Verified
- Description
- Nuclear envelope/ER integral membrane protein; interacts and functions with Brr6p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA export from the nucleus and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism 1 2 3 4
- Name Description
- APical growth revealed by Quantitative morphological analysis 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 138
- Mol. Weight (Da)
- 16511.8
- Isoelectric Point
- 9.27
- Median Abundance (molecules/cell)
- 2755 +/- 1137
- Half-life (hr)
- 17.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all APQ12 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Integral membrane protein of the nuclear envelope and ER; involved in lipid homeostasis; role in nuclear export of mRNA, and nuclear envelope organization
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in lipid homeostasis (IMP, IGI)
- involved in nuclear envelope organization (IMP)
Cellular Component
- Manually Curated
- located in endoplasmic reticulum (HDA, IDA)
- located in membrane (IDA)
- located in nuclear envelope (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has a large cell size with abnormally elongated bud, and an anaphase delay; null mutant is heat and cold sensitive, slow growing at permissive temperature and has competitive fitness defects; null mutant is sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents, accumulating sterol precursors and monounsaturated fatty acids at reduced temperatures; null mutant is defective in the nuclear export of poly(A)+ mRNA, mislocalizes nuclear pore complex (NPC) cytoplasmic filament proteins and mRNA export factor, Dbp5p; null mutant has a vacuolar fragmentation deficiency, and nuclear morphology defects with abnormal nuclear envelope and NPC distribution; null mutant displays filamentous growth defects and is unable to undergo invasive growth; null mutant has decreased chronological lifespans, decreased acquired thermotolerance and does not accumulate trehalose after heat shock; null mutant is sensitive to metal treatment and antifungals
Classical Genetics
- null
- autophagy: decreased
- cell cycle progression: abnormal
- cell shape: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- cold sensitivity: increased
- nuclear export: decreased
- nuclear morphology: abnormal
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- RNA accumulation: increased
- RNA modification: increased
- vegetative growth: decreased rate
- overexpression
- null
- acid pH resistance: decreased
- acquired thermotolerance: decreased
- bud morphology: abnormal
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- colony sectoring: increased
- competitive fitness: decreased
- competitive fitness: increased
- fermentative growth: decreased rate
- filamentous growth: decreased
- heat sensitivity: increased
- invasive growth: absent
- killer toxin resistance: decreased
- metal resistance: decreased
- oxidative stress resistance: decreased
- protein activity: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- reticulophagy: decreased
- toxin resistance: decreased
- transposable element transposition: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
846 total interactions for 510 unique genes
Physical Interactions
- Affinity Capture-MS: 4
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 3
- Co-localization: 1
- PCA: 1
Genetic Interactions
- Dosage Rescue: 2
- Negative Genetic: 557
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 1
- Positive Genetic: 193
- Synthetic Growth Defect: 49
- Synthetic Lethality: 21
- Synthetic Rescue: 9
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.