ULP2 / YIL031W Overview


Standard Name
ULP2 1
Systematic Name
YIL031W
SGD ID
SGD:S000001293
Aliases
SMT4 6
Feature Type
ORF , Verified
Description
Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins; plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate; human homolog PML implicated in promyelocytic leukemia can partially complement yeast null mutant 1 2 3 4 5
Name Description
UbL-specific Protease 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
1034
Mol. Weight (Da)
116875.8
Isoelectric Point
6.83
Median Abundance (molecules/cell)
454 +/- 235

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all ULP2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
SUMO protease involved in compaction of dispersed interphase chromatin into chromosomes, mitotic cell cycle spindle assembly checkpoint that delays the metaphase/anaphase transition until the spindle is correctly assembled with chromosomes attached, and maintenance of the integrity of extrachromosomal plasmid DNA

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant is extremely slow-growing and displays enlarged cells with elongated buds and abnormal spindles, irregularly shaped colonies, higher rates of chromosome loss, and sensitivity to UV, gamma rays, heat, MMS, and HU; conditional allele confers resistance to doxorubicin; in large-scale studies, null mutant scored as inviable; repression confers lowered competitive fitness; diploid heterozygous null mutant has lower innate thermotolerance and sensitivity to myriocin
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast ULP2 is homologous to human PML, and has been used to study acute promyelocytic leukemia

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


489 total interactions for 357 unique genes

Physical Interactions

  • Affinity Capture-MS: 227
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 6
  • Biochemical Activity: 6
  • Co-crystal Structure: 3
  • Co-localization: 1
  • Co-purification: 5
  • PCA: 91
  • Protein-peptide: 2
  • Reconstituted Complex: 10
  • Two-hybrid: 7

Genetic Interactions

  • Dosage Growth Defect: 3
  • Dosage Lethality: 1
  • Dosage Rescue: 17
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 26
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 17
  • Synthetic Rescue: 43
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
6
Targets
1
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
46
Additional
37
Reviews
24

Resources