NMD2 / YHR077C Overview

Standard Name
NMD2 1
Systematic Name
IFS1 , UPF2 3 , SUP111 6 , SUA1 7
Feature Type
ORF , Verified
Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; acts as a scaffold protein bridging Nam7p and Upf3p; involved in telomere maintenance; contains an acidic domain and three middle domains of eukaryotic initiation factor 4G (mIF4G) with aspartic 59 of mIF4G-1 important for NMD activity and translation termination accuracy 1 2 3 4 5
Name Description
Nonsense-mediated MRNA Decay 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

NMD2 is located on the right arm of chromosome VIII between PTC7 type 2C serine/threonine protein phosphatase and high osmolarity-regulated gene YHR078W; coding sequence is 3383 nucleotides long with an intron at 7..119, a ~150-nucleotide microsatellite toward the C-terminus, and 12 other SNPs, 6 of which cause amino acid polymorphisms
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Nmd2p is 1089 amino acids long, very long-lived, low in abundance; has 2 armadillo-type folds; phosphorylated on 3 serines
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
2350 +/- 1042
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all NMD2 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Protein involved in DNA recombination and 3'-5' exonucleolytic nonsense-mediated decay; localizes to cytoplasm and polysomes

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null displays slow growth, small defect in vacuolar fragmentation, large nucleolus, short telomeres, decreased sporulation, decreased silencing at the silent mating type locus HMR, decreased Ty1 mobility, decreased competitive fitness, resistance to antifungals, cisplatin, MMS, and caffeine, sensitivity to camptothecin and cycloheximide, decreased oxidative stress resistance, decreased chronological lifespan
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Nmd2p interacts physically with proteins involved in rRNA processing; NMD2 interacts genetically with genes involved in transcription

533 total interactions for 368 unique genes

Physical Interactions

  • Affinity Capture-MS: 76
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 10
  • Co-localization: 1
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 13

Genetic Interactions

  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 266
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 3
  • Positive Genetic: 99
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 2
  • Synthetic Rescue: 30
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

NMD2 promoter is bound by Abf1p
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2024-06-24

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.