UBC9 / YDL064W Overview


Standard Name
UBC9 1
Systematic Name
YDL064W
SGD ID
SGD:S000002222
Feature Type
ORF , Verified
Description
SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) 1 2 3
Name Description
UBiquitin-Conjugating 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
157
Mol. Weight (Da)
17917.6
Isoelectric Point
9.65
Median Abundance (molecules/cell)
5656 +/- 2991
Half-life (hr)
9.6

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all UBC9 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
SUMO transferase involved in conjugation of the SUMO protein modifier to target proteins; required for spindle elongation during mitosis; localizes to condensed nuclear chromosomes

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene; conditional mutant arrests at the G2/M phase transition at non-permissive temperature with large-budded cells containing a single nucleus, short spindle, and replicated DNA; conditional mutant has increased resistance to hyperosmotic stress, decreased resistance to amino acid starvation, and elevated levels of protein sumoylation at permissive temperature; conditional and repressible mutants display elevated levels of chromosome loss and gross chromosomal rearrangements
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast UBC9 is homologous to human UBE2I, and has been used to study cancer

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


440 total interactions for 294 unique genes

Physical Interactions

  • Affinity Capture-MS: 98
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 5
  • Biochemical Activity: 28
  • Co-crystal Structure: 2
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 46
  • Two-hybrid: 47

Genetic Interactions

  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Negative Genetic: 144
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 2
  • Positive Genetic: 22
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 14
  • Synthetic Rescue: 7
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
2
Targets
2
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
63
Additional
99
Reviews
38

Resources