CDC28 / YBR160W Overview

Standard Name
CDC28 1 2
Systematic Name
CDK1 , HSL5 , SRM5 63
Feature Type
ORF , Verified
Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates metabolism, basal transcription, chromosome dynamics, growth and morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can complement yeast conditional cdc28 mutants; human CDK1, CDK2 can complement yeast cdc28 null mutant 3 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Name Description
Cell Division Cycle 4
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

CDC28 is located on the right arm of chromosome II between IFA38 microsomal beta-keto-reductase and CSH1 mannosylinositol phosphorylceramide synthase; coding sequence is 897 nucleotides long with 2 SNPs, both of which are silent
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Cdc28p is 298 amino acids long, of average half-life, low abundance; phosphorylated on 9 residues
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
6670 +/- 1539
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all CDC28 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Protein serine/threonine kinase subunit of multiple cyclin-dependent kinase (CDK) complexes, which regulate the mitotic and meiotic cell cycles as well as many other cellular processes; CDK complexes function at various locations throughout the cell

View computational annotations

Biological Process

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Essential gene; conditional mutants display allele specific cell cycle arrest as either large, unbudded, conjugation-competent cells with a single, undivided nucleus and pear-shaped morphology at START in the G1 phase or as abnormally large, elongated cells with undivided nuclei at the G2/M phase transition; reduction-of-function mutant has a decreased rate of growth and buds in a bipolar or unipolar manner; conditional mutants are benomyl sensitive, have an elevated rate of plasmid and chromosome loss, and accumulate trigylcerides; homozygous diploid mutants have a sporulation defect
Disease Details


Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.

Yeast CDC28 is homologous to human CDK1 and CDK2, and has been used to study cancer

Manually Curated

Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Cdc28p interacts physically with proteins involved in mitosis; CDC28 interacts genetically with genes involved in transcription

2657 total interactions for 1572 unique genes

Physical Interactions

  • Affinity Capture-MS: 327
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 75
  • Biochemical Activity: 538
  • Co-localization: 6
  • Co-purification: 10
  • PCA: 47
  • Protein-peptide: 2
  • Proximity Label-MS: 2
  • Reconstituted Complex: 50
  • Two-hybrid: 36

Genetic Interactions

  • Dosage Growth Defect: 6
  • Dosage Lethality: 397
  • Dosage Rescue: 48
  • Negative Genetic: 654
  • Phenotypic Enhancement: 63
  • Phenotypic Suppression: 30
  • Positive Genetic: 113
  • Synthetic Growth Defect: 168
  • Synthetic Lethality: 40
  • Synthetic Rescue: 42
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Transcription is downregulated by Spt10p; transcription is upregulated by Ixr1p during hypoxic response; transcription is regulated by Med4p in response to heat and by Sfp1p in response to stress
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2006-08-09

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.