TPM1 / YNL079C Overview


Standard Name
TPM1 1
Systematic Name
YNL079C
SGD ID
SGD:S000005023
Feature Type
ORF , Verified
Description
Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication 1 2 3 4
Name Description
TroPoMyosin 1
Paralog
TPM2 4
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
TPM1 has a paralog, TPM2, that arose from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
199
Mol. Weight (Da)
23515.8
Isoelectric Point
4.28
Median Abundance (molecules/cell)
29745 +/- 11551
Half-life (hr)
12.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all TPM1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Actin filament binding protein involved in actin filament organization and bundle assembly; has role in actin filament reorganization during cellular hyperosmotic response; involved in mitotic actomyosin contractile ring assembly, filamentous growth and pseudohyphal growth; localized to actin filament, actin filament bundle, and bud neck contractile ring

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The tpm1 null mutant is viable; the null mutant of paralog tpm2 is viable; the tpm1 tpm2 double mutant displays negative genetic interactions.

1052 total interactions for 698 unique genes

Physical Interactions

  • Affinity Capture-MS: 25
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 1
  • Co-localization: 1
  • Co-purification: 1
  • Proximity Label-MS: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 2

Genetic Interactions

  • Dosage Rescue: 7
  • Negative Genetic: 733
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 12
  • Positive Genetic: 179
  • Synthetic Growth Defect: 21
  • Synthetic Lethality: 28
  • Synthetic Rescue: 21
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
10
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
47
Additional
58
Reviews
28

Resources