DSK2 / YMR276W Overview


Standard Name
DSK2 1
Systematic Name
YMR276W
SGD ID
SGD:S000004889
Feature Type
ORF , Verified
Description
Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; K48-Ub chain binding protein that also binds K63-linked chains in vitro; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; contains an N-terminal Ub-like (UBL) and a C-terminal Ub-associated (UBA) domain; protein abundance increases in response to DNA replication stress 2 3 4 5 6 7 8 9
Name Description
Dominant Suppressor of kar1 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
DSK2 is located on the right arm of chromosome XIII between BUL1 alpha-arrestin and FCP1 CTD phosphatase; coding sequence is 1122 nucleotides long with 12 SNPs, 4 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Dsk2p is 373 amino acids long, somewhat longer lived, and low in abundance; contains 2 ubiquitin-like domains and 2 chaperonin-binding domains; protein abundance increases in response to DNA replication stress; acetylated on K52, phosphorylated on 5 residues, ubiquitinylated on 5 lysines
Length (a.a.)
373
Mol. Weight (Da)
39334.2
Isoelectric Point
4.56
Median Abundance (molecules/cell)
6259 +/- 3581
Half-life (hr)
11.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all DSK2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Nuclear protein involved in spindle pole body duplication, ER-associated ubiquitin-dependent protein catabolism, and vacuolar protein localization

View computational annotations

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant shows delayed degradation of reporter proteins; overexpression leads to increased accumulation of polyubiquitin and cell cycle arrest; in systematic studies mutants exhibit reduced competitive fitness, and increased sensitivity to flucytosine and Congo Red; mutant diploids are auxotrophic for myo-inositol
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast DSK2 is homologous to human UBQLN2 and has been used to study amyotrophic lateral sclerosis type 15
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Dsk2p interacts physically with proteins involved in protein catabolism; DSK2 interacts genetically with genes involved in transcription

239 total interactions for 136 unique genes

Physical Interactions

  • Affinity Capture-MS: 21
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 31
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-purification: 1
  • Far Western: 1
  • Reconstituted Complex: 33
  • Two-hybrid: 16

Genetic Interactions

  • Dosage Growth Defect: 10
  • Dosage Lethality: 3
  • Dosage Rescue: 2
  • Negative Genetic: 59
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 7
  • Positive Genetic: 14
  • Synthetic Growth Defect: 11
  • Synthetic Rescue: 9
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
DSK2 promoter is bound by 13 different transcription factors in response to heat; DSK2 transcription is regulated by Sfp1p in response to stress
Regulators
18
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
44
Additional
44
Reviews
28

Resources