MRC1 / YCL061C Overview

Standard Name
MRC1 1
Systematic Name
Feature Type
ORF , Verified
S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; exposure to ethanol affects localization; mammalian claspin homolog 2 3 4 5 6 7 8 9
Name Description
Mediator of the Replication Checkpoint 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
1302 +/- 378


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all MRC1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Protein involved in the DNA replication checkpoint, the intra-S DNA damage checkpoint, and regulation of DNA replication during the osmotic stress response; involved in mitotic sister chromatid cohesion, replication fork protection, DNA repair, chromatin silencing, and maintenance of DNA repeat elements and telomeres; subunit of the replication fork protection complex; localizes to nuclear replication fork and nuclear periphery

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant shows increased duration of the S phase of the cell cycle and increased chromosome instability; null mutation results in constitutive phosphorylation of Rad53p; null mutants are sensitive to some chemicals, including benomyl, hydroxyurea, MMS, myriocin, quinine, but show increased resistance to other chemicals, including camptothecin, fluconazole, mycophenolic acid; null mutation increases transposition of Ty elements
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

1289 total interactions for 484 unique genes

Physical Interactions

  • Affinity Capture-MS: 38
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 54
  • Biochemical Activity: 11
  • Co-fractionation: 1
  • Proximity Label-MS: 2
  • Reconstituted Complex: 12
  • Two-hybrid: 9

Genetic Interactions

  • Dosage Growth Defect: 8
  • Dosage Lethality: 3
  • Dosage Rescue: 10
  • Negative Genetic: 648
  • Phenotypic Enhancement: 57
  • Phenotypic Suppression: 25
  • Positive Genetic: 65
  • Synthetic Growth Defect: 182
  • Synthetic Lethality: 113
  • Synthetic Rescue: 47
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.