HO / YDL227C Overview


Standard Name
HO 1
Systematic Name
YDL227C
SGD ID
SGD:S000002386
Feature Type
ORF , Verified
Description
Site-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p 2 3 4 5
Name Description
HOmothallic switching endonuclease 3 5
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
HO is on the left arm of chromosome IV between replication origin ARS404 and GCS1 GTPase activating protein; coding sequence is 1761 nucleotides long with 11 SNPs, 5 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Ho protein is 586 amino acids long, contains 5 inteins, ubiquitinylated on 4 lysines
Length (a.a.)
586
Mol. Weight (Da)
66118.3
Isoelectric Point
9.56

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all HO alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
DNA endonuclease involved in mating type switching; catalyzes the formation of a double-stand break at the mating type locus to initiate a gene conversion event

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null has increased replicative lifespan, decreased competitive fitness; heterozygous null is haploinsufficient
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Ho protein interacts physically with proteins involved in protein catabolism; HO interacts genetically with genes involved in mitotic cell cycle, meiotic cell cycle, and DNA repair

46 total interactions for 30 unique genes

Physical Interactions

  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 11
  • Affinity Capture-Western: 12
  • Co-crystal Structure: 1
  • Co-purification: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 2

Genetic Interactions

  • Dosage Growth Defect: 1
  • Negative Genetic: 10
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
HO promoter is bound by Swi4p and Swi6p during mitotic G1; HO transcription is downregulated by homeodomain mating type protein alpha2 during mitotic cell cycle; HO transcription is upregulated by Snf2p, and by Nrs1p during nitrogen starvation response
Regulators
12
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2005-12-13

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
79
Additional
285
Reviews
53

Resources