EAP1 / YKL204W Overview


Standard Name
EAP1 1 2
Systematic Name
YKL204W
SGD ID
SGD:S000001687
Feature Type
ORF , Verified
Description
eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade 1 2 3 4
Name Description
EIF4E-Associated Protein 1 2
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
EAP1 is located on the left arm of chromosome XI toward the telomere and between LOS1 nuclear pore protein and replication origin ARS1103; coding sequence is 1899 nucleotides long with 10 SNPs, 4 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Eap1p is 632 amino acids long, shorter-lived, low in abundance; phosphorylated on 37 residues
Length (a.a.)
632
Mol. Weight (Da)
69779.2
Isoelectric Point
10.16
Median Abundance (molecules/cell)
4877 +/- 1412
Half-life (hr)
8.2

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all EAP1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
mRNA and eIF4E binding protein involved in deadenylation-dependent decapping of nuclear mRNA, mRNA catbolic process and negative regulation of translation; localizes to stress granule and polysone

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant shows decreased utilization of ammonium sulfate as nitrogen source, increased levels of unprocessed 35S pre-rRNA and decreased growth rate; reduction of function results in decreased autophagy under nitrogen starvation; in systematic studies, mutants show decreased anaerobic growth, increased levels of some amino acids, decreased endocytosis and sensitivity to benomyl, rapamycin (sirolimus), hydroxyurea, DMSO, Congo Red; overexpression causes increased mutation frequency
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Eap1p interacts physically with proteins involved in translation; EAP1 interacts genetically with genes involved in transcription

562 total interactions for 506 unique genes

Physical Interactions

  • Affinity Capture-MS: 153
  • Affinity Capture-RNA: 9
  • Affinity Capture-Western: 14
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • PCA: 1
  • Proximity Label-MS: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions

  • Dosage Lethality: 2
  • Dosage Rescue: 3
  • Negative Genetic: 181
  • Phenotypic Enhancement: 1
  • Positive Genetic: 147
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 18
  • Synthetic Rescue: 8
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
EAP1 transcription is regulated by Sfp1p in response to stress; Eap1 protein activity is regulated by protein phosphatase PP2A, and by Bcy1p and Snf1p in reponse to glucose starvation
Regulators
4
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
30
Additional
31
Reviews
12

Resources