ChIP-Seq of constitutively expressed Ndt80-Myc in S. cerevisiae

Dataset: ChIP-Seq of constitutively expressed Ndt80-Myc in S. cerevisiae

External ID: GSE90660
Reference: Nocedal I, et al. (2017)
Channels: 1
Conditions: 6
Description: The purpose of this experiment was to identify the genes bound by Ndt80 in S. cerevisiae when constitutively expressed, for the native Ndt80 as well as a heterologous Ndt80 from Pichia pastoris. For the ChIPs on the native Ndt80s, Ndt80 was tagged with c-myc; for the heterologous ChIP P. pastoris Ndt80 was integrated into the S. cerevisiae genome and tagged with c-myc. In both cases, the native Ndt80 promoter was also replaced with an A. gossypii Gal1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown overnight in SRaffinose until log-phase, then in SRaffinose + 1.7% Galactose for 5 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.
Categories: transcriptional regulation

Conditions

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